Do you have experiences in extracting DNA from cleared nematodes? Which methods can you suggest?
Hi Mohsen,
I can suggest an efficient method describe by Della Porta et al., 1983 Plant Mol. Biol. Rep. 1, pp. 19-21 for plant DNA miniprep but useful also for nematodes.
Regards
Dear All
I am doing PCR on a COX II gene on a nematode using the followings primers
COXII F TCT AGT TAT ATA GAT TGR TTT YAT
COXII R CAC CAA CTC TTA AAA TTA TC
It is mention to cite I have to use this primers to get a 629 bp Band the problem
that I face back to the primers as my PCR just work well for less than 10 days and after that period I fail to run a successful PCR . How can I elevate the life of primers I recently change my company an ordered a new one but unfortunately the problem has persist to me yet. I work with ITS related primers in parallel but never faced such problem.
PS: I used DNA free water to wet my primers as company ordered.
سلام آقاي نجاري
من هم براي استخراج دي ان اي از نمونه هاوي شفاف شده با لاكتوفنول مشكل دارم. شما از اين روش پيشنهاد شده استفاده كرديد؟ آيا جواب خوبي گرفتيد؟
با تشكر
Please search online protocol for extraction of DNA from phenolic samples
you shold add other material to your lysate buffer to eleminate
Extera phenole.the last protocol was not fair to my extraction.
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