I want to analyze the stable isotopic discrimination of carbon and oxygen from tree ring cellulose. Can somebody help me how to extract alpha cellulose from tree rings (increment cores) and analyzing isotopic discrimination of C and O with best one protocols.
Hello,
For C: I used the Brendel and water-modified Brendel standard laboratory procedure, to extract alpha cellulose from tree rings and it worked very nice.
You can start by having a look at these publications:
1. Brendel O, Iannetta PPM, Stewart D (2000) A Rapid and Simple Method to Isolate Pure Alpha-cellulose. Phytochemical Analysis 11, 7-10.
2. Gaudinski JB, Dawson TE, Quideau S, Schuur EAG, Roden JS, Trumbore SE, Sandquist DR, Oh SW, Wasylishen RE (2005) Comparative Analysis of Cellulose Preparation Techniques for Use with 13C, 14C, and 18O Isotopic Measurements. Analytical Chemistry 77, 7212-7224.
Cheers,
Ana
We use the Loader modified Jayme-Wise method. There is a good recent discussion of the many methods available here http://treephys.oxfordjournals.org/content/25/5/563.full.pdf
The Loader method is discussed here
Loader, N.J., I. Robertson, A.C. Barker, V.R. Switsur and J.S. Waterhouse.
1997. An improved technique for the batch processing of
small wholewood samples to
α-cellulose. Chem. Geol. 136:
313–317.
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