I have urine samples collected from treated animals, which i want to analyse on HPLC. How can I extract the BPA?
I would advise you to use LCMS of GCMS rather than HPLC as the detection limit for a normal HPLC may not be very useful for measuring urine levels of BPA as the levels may sometimes be too low for detection by HPLC. Of course the best way is to use LCMSMS or GCMSMS.
Please have a look at our paper as follows: Urinary Bisphenol A Concentrations and Their Implications for Human Exposure in Several Asian Countries. Environ.Sci. Technol. 2011, 45, 7044–7050.
Hi Naga
QUANTITATION OF BPA IN BIOLOGICAL SAMPLES CAN BE DONE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY.
Follow this link for more details :
http://dmd.aspetjournals.org/content/33/11/1748.full.pdf+html
GUD LUCK
Regards
I would advise you to use LCMS of GCMS rather than HPLC as the detection limit for a normal HPLC may not be very useful for measuring urine levels of BPA as the levels may sometimes be too low for detection by HPLC. Of course the best way is to use LCMSMS or GCMSMS.
Please have a look at our paper as follows: Urinary Bisphenol A Concentrations and Their Implications for Human Exposure in Several Asian Countries. Environ.Sci. Technol. 2011, 45, 7044–7050.
Thank You Dr. Alok and Dr. Mustafa. Actually I am planning to analyze the BPA in treated animal's urine and feces samples by HPLC and MALDI-TOF.
My question is the solvent which we use for the extraction will have the effect on chromatogram, the solvent SHOULD be the same as solvent syntem we use. or it can be any one of the solvent from the solvent system?
I'd recommend that you use SPE rather than LLE for your sample prep. That way you avoid the issue of co-extracted materials. Not sure why you'd want to go through the MALDI step - you should have excellent sensitivity for BPA with either ESI or APCI.
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