The experiment involves a cancer cell line. So that I can normalize the experiment properly, I need DNA extraction from the sample, alongside with the RNA extraction results. Both extractions must come from the same sample. Is there a recommended kit or protocol?
If it's a cell line, then just...split the cells?
Grow cells, trypsinise, collect, resuspend in a small volume, take half of that for RNA, half for DNA? Unless you're dealing with countable numbers of cells (
I would also recommend splitting the cells. If you have a homogenous single cell suspension and you take equal volumes at the same time for DNA extraction and RNA extraction, this is probably the best way to do the experiment. Extracting DNA and RNA from the exact same cells is possible, but more involved, and I wouldn't say it's BETTER unless you have very very few cells to work with.
If you need to avoid trypsin, you can use a lysis reagent that's compatible with both DNA and RNA extraction, and then split the lysate.
Qiagen sells a kit for getting DNA and RNA from a single sample. You start with the same sample material, then the workflow splits into two preps, one for DNA and one for RNA. It is meant for animal tissues and cells so it should work well for your cancer cell lines.
AllPrep DNA/RNA Micro, Mini, and 96 Kits - QIAGEN
Thermofisher's (Invitrogen) TRizol reagent can do that from the same sample. Follow the manufacturers protocol. It works well in our hands.
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