How can I explain a dramatical decrease of PARP?

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Paola Di Bonito

The detection limit of your antibody?

Rashadul Islam

Is PARP measured in constant temperature??

Nadia Mustapha

@Paola: I didn't understand your question

@Islam: yes, in the same conditions.

You should keep the mind in binding affinities with measuring solvents. when stable, only then measurement should perform.

thanks for your answer. everything is stable, but i always find such result and i just need to find an explanation for that. why a dramatic decrease when there is not a great difference between 40 and 50 µg/ml of tested sample.