How can I explain a dramatical decrease of PARP?

More Nadia Mustapha's questions See All

Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

How can I adapt cyclic server model in hospital operations?

To help enchancing patients flow and service efficiency

18 July 2024 482 2 View

Are the messages transmitted in classical channel between participants in quantum cryptographic protocols free from being blocked or delayed ?

In quantum cryptographic protocols, participants typically share both a quantum channel and a classical authenticated channel. Authenticated channels ensure that messages come from legitimate...

22 June 2024 7,074 3 View

What Would You Do, Wall Street Journal wsj.com?

for systemic risk of wjs.com experience best experienced alongside other explorer spirit independently in creativity.

03 May 2024 6,821 1 View

What is a constant touchstone or supercomputer sims for Elizabeth Holmes?

Constant touchstones following the protocol introduces a new dimension of bioethics to the bioinformatics about big ideas, modes of inquiry, intellectual traits and habits of mins but it is...

24 April 2024 8,739 3 View

Could you please guide me how can I manually separate genomic and plasmid DNA from algae simultaneously?

Next step is doing qPCR. Thanks

23 April 2024 5,442 5 View

Hi, I am going to isolate genomic and plasmid DNA from algea simultaneously and manually, Do you have any suggestion?

I found a kit that they separated plasmid and genomic DNA form mammalian cell, but I want to extract both plasmid and genomic DNA manually. I also want to use the extracted DNA for qPCR.

23 April 2024 1,857 1 View

Welche Effekt hat die Diabetesedukation durch Krankenschwestern bei Diabetes Typ 2 Patienten in Primärversorgungszentren?

„Welche Effekt hat die Edukation von strukturierte Schulungsprogramme, wie Disease-Management-Programme (DMPs), durch geschultes Pflegepersonal auf den glykosyliertes Hämoglobin-Wert (HbA1c) und...

14 April 2024 248 2 View

Why am I getting low isotopic enrichment in my expressed protein even though I am using labeled media?

I am expressing a membrane protein (about 15 kDa) in BL21(DE3) cells. The expression is good, and the yield is decent, but each time I checked the mass with a TOF mass spec, the mass I get is...

11 April 2024 7,169 3 View

Do you have references dedicated to the influence of batch for the same steel with the same process and the same composition?

I am trying to find papers dedicated to the effect of a change of supplier or batch on the variation in the tensile strength of the steel.thank you

04 April 2024 1,897 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

For a case-control study for Breast Cancer incidents in a particular County in USA, what data can be used as Control? E.g. colorectal cancer?

The County health department is providing Breast cancer incident data. If colorectal cancer cases could be used as control, then we can get respective data from health department as well.

22 July 2024 9,954 4 View

How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View

Paola Di Bonito

The detection limit of your antibody?

Rashadul Islam

Is PARP measured in constant temperature??

Nadia Mustapha

@Paola: I didn't understand your question

@Islam: yes, in the same conditions.

You should keep the mind in binding affinities with measuring solvents. when stable, only then measurement should perform.