from 40 to 50 µg/ml of tested concentration of sample, I have a dramatical decrease of PARP. how can I explain that?
The detection limit of your antibody?
Is PARP measured in constant temperature??
@Paola: I didn't understand your question
@Islam: yes, in the same conditions.
You should keep the mind in binding affinities with measuring solvents. when stable, only then measurement should perform.
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