How can I evaluate the cell viability using the dye exclusion method (Fast Green) on adhered cells?

More Catia S Branco's questions See All

RTgutGC cells. Does anyone know a reliable provider for these cells?

I cannot seem to find a source that sells these cells… And the papers I've read so far a not clear concerning cell line origin. It's always someone else that gave them a vial. Assinante is highly...

09 April 2023 3,159 0 View

Is this an interesting study about pneumonia for your project?

Curr Opin Crit Care. 2018 Feb;24(1):49-54. doi: 10.1097/MCC.0000000000000469. The microbiome of the critically ill patient. Akrami K1, Sweeney DA. Author information Abstract PURPOSE OF REVIEW:...

06 September 2018 5,582 1 View

Which is the best definition of frailty in the elderly?

some scores for fragility

03 May 2018 1,642 6 View

Are hospital susfacesor devices reservoirs for drug resistant pathogens , how long?

Are hospital susfaces devices reservoirs for drug resistant pathogens , how long?

23 October 2017 9,766 3 View

Sometimes reverse shoulder replacement requires glenoid bone-grafting.Is there any way to measure the glenoid bone density/predict the need for graft?

We would like to get a way to predict the need for glenoid bone-grafting in a reverse shoulder replacement planning

18 April 2017 4,339 6 View

How to deal with INSPIRE voidable attributes using HALE?

I'm harmonizing Elevation vector data with HALE. In the INSPIRE schema for this theme there is alot of voidable attributes that I don't have information for. I read on the INSPIRE forum that to...

18 October 2016 8,142 1 View

Any suggestion for a good antibody against human CD38?

Can someone suggest me an antibody suitable for WB analysis to detect human CD38? Thank you!

04 October 2016 7,174 11 View

What's the name of distal phalanx pseudarthrosis, due to interposition of ungueal matrix?

17 September 2016 8,006 3 View

An increased biceps effort can result in a SLAP lesion or a tear of its tendon at the elbow. Why does it happen one or the other?

- Are there different risk factors for each one? - Why some people have one or the other lesion? - What are the main etiological differences?

14 October 2015 9,463 4 View

Is it possible to freeze the samples after the purification step but before the IEF process for Proteomic analysis?

I'm doing Proteomic analysis and did all the steps of the purification process on my protein samples, but then I realized I only need 4 samples for the IEF process. Is it possible to re-freeze the...

17 February 2014 3,037 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Steingrimur Stefansson

Hi Catia, as far as I can tell, Fast Green works the same way as Trypan Blue.

http://cancerres.aacrjournals.org/content/43/1/258.full.pdf

https://www.bcbsri.com/sites/default/files/polices/In_Vitro_Chemoresistance_and_Chemosensitivity_Assays.pdf

Catia S Branco

Hi Steingrimur Stefansson, thank you for your attention.