Hi everyone Can anyone helps me by providing methodology for estimating beta 1,3 glucanase and chitinase from wheat root extracts prepared with buffer..
One of the most standard methods for estimating chitinase assay is by Resig et al. 1955. For beta1,3-glucanase- native PAGE is better. refer to buchenauer et al1995.
I am assuming you want to measure enzyme activity for chitinase and glucanase. If so, there are dye labeled substrates for each that can be used in a simple colorometric assay. Loewe Biochemica, Munich, Germany
McCollum, T. G., Doostdar, H., Mayer, R. T. and McDonald, R. E. 1997. Characterization of chitinases and P-1,3-glucanases in grapefruit flavedo during fruit development. Physiol. Plant. 99: 486494.
Thank you Rohit and Greg for your time and suggestion. Yes, Greg you are right I am going for measuring enzyme activity. I am finding the paper you recommended interesting.