I`m now doing some research about GIST(human gastrointestinal stromal tumour), and there is a big problem that I have failed to establish GIST exograft animal model. 1×107 GIST T1 cell line were given hypodermic injection on nude mouse, after 10 days, tumor was failed to growth. Some one who have successful established this exograft model could give me advice, thanks.
You have to know the importance of where tumor cells are transplanted. GIST occurs from Interstitial cells of Cajal (ICC) with active mutation of c-KIT, the receptor of SCF. That is why the hypodermic injection is very artificial and difficult for GIST T1 cell line to form xenograft tumor tissue. Of course, so many kinds of tumor cell lines are able to form xenograft tumor under the skin of nude mice, but it has been widely accepted that the orthotopical transplantation is much better to reflect the tissue-dependent characteristics of each tumor. Why not try to transplant G1 cells into the gastric or intestinal mucosa?
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