How can I encapsulate FITC-BSA or FITC-dextran in a polymer matrix (as for example chitosan)?

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How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...

21 February 2021 5,614 3 View

Simulation or Experiment? How do we choose?

If we want to assess if a phenomenon is going in a direction we expect, we can simulate it, using digital models, possibly agent models factoring in known properties and interaction features. We...

12 February 2021 1,141 21 View

Matrix based scientific data analysis: extrapolating to matrices of sets?

Data sets, when structured, can be put in vector form (v(1)...v(n)), adding time dependency it's v(i, t) for i=1...n and t=1...T. Then we have a matrix of terms v(i, j)... Matrices are important,...

30 January 2021 9,295 6 View

How to calculate the gas composition for a heat treatment where a very low oxygen partial pressure is needed?

I'm interested in a selective oxidation in an austenitic stainess steel so that Chromia rather then ferrous oxide is formed on top of my metal. I know I should have a value of pO2 around...

16 December 2020 8,541 7 View

The loss of hyperbolicity in PDE?

Question about the loss of hyperbolicity in nonlinear PDE: when complex eigenvalues appear, what is the effect on flow? I understand that we do not have general results on existence in this case,...

16 December 2020 2,571 3 View

How to resolve the sample contamination issue in transmission electron microscopy (TEM) even after long-time plasma cleaning?

Hi, I am doing TEM routinely in my daily research work. Most of my TEM samples are the inorganic oxide-metal nanocomposite films deposited on single crystal oxide substrate (such as STO, MgO)....

11 December 2020 2,695 5 View

Human-Machine co-operations or competition? Where are we today?

At the cross-roads of Technology for Advanced Manufacturing, involving robots and humans, and Digitised Economic Sectors, is a governance matter for Cognitive Manufacturing [1] and rules of the...

21 November 2020 7,145 20 View

LIFE in SMART CITIES: Animal data versus Human data, complementarity or not?

One observation is that in any city (those I know) you have humans, of course, but you also have many animals. Statistics of humans start with population data (for instance demographics) and...

08 November 2020 4,127 6 View

Sample Size in Count analysis ?

Hello everybody and thank you in advance. I would like to ask you about a power analysis problem starting from an example. I have two medical devices (A and B) that finds lymphnodes. My...

01 November 2020 4,306 11 View

How to find a sequence in a mouse gene?

I need the sequence corresponding -3,149 and -3,024 relative to the translational start site of my interest gene. I don't have other info. Can someone help me to understand what I have to...

26 October 2020 325 2 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Daniel Oladapo Olaniregun

Aseptic packaging in sterile environment using inner material pack.

Yes if it will not cost me too much me. Let me first see the scale of what the researcher is handling

David Jensen

This publication may be of help:

https://www.researchgate.net/publication/260373640_Controlling_chitosan-based_encapsulation_for_protein_and_vaccine_delivery

Article Controlling chitosan-based encapsulation for protein and vac...