How can I encapsulate FITC-BSA or FITC-dextran in a polymer matrix (as for example chitosan)?

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Problems with Sybr green melting curve?

Hello, Do you have experience with this type of melting curve? Can anyone explain to me why there is a lowering of the curve? I'm using SYbr select master mix on quant studio 5 real time pcr.

21 May 2024 1,191 0 View

Noves Ricerca Group?

Good morning to all, I have received an invitation to publish a paper from Noves Ricerca Group in a Q2 Journal. They have read my paper and very quickly they have accepted it for publication. It...

20 May 2024 7,407 3 View

What type of high-quality nanopure water do you use for NTA analyses?

We recently acquired a ViewSizer3000 from Horiba, and the manufacturer recommended us to use nanopure water from XZero for the NTA analyses. Do you recommend using any other brands of NW? Which...

05 May 2024 1,976 1 View

What is the best way to concentrate the protein and remove the imidazole post Ni-NTA purification?

I've always struggled with concentrating and washing my proteins after purification. Often I lose almost everything in the process. I have used dialysis membranes followed by column filter...

16 April 2024 5,716 0 View

Orbitrap Astral Mass Spectrometer: applications?

Hi everyone, I'm a PhD student at CAST, Analytical Biochemistry and Proteomics Research Unit, University, "G. d'Annunzio" of Chieti-Pescara. We currently use the Thermo Fisher Orbitrap Fusion...

10 April 2024 9,346 0 View

Has anyone experience with a good one antibody for the western blot detection of SQSTM1 in human cells?

The old antibody has been discontinued and the currently offered are detecting a band at 62 kDa, which does not reflect the correct molecular weight (47 kDa).

17 March 2024 4,300 2 View

What can be the causes of which protein bands don't run through a SDS-PAGE gel?

I'm having a problem with SDS-gel. I cannot get the protein bands to run fully on the gel. For me it's the first time with SDS-gel. I prepared a polyacrylamide gel with 5% stackyng gel and 15%...

10 March 2024 228 2 View

Seeking Reviews for Q-TOF LCMS-9030 Mass Spectrometer?

Dear Forum Members, I hope this message finds you well. I am currently considering the purchase of a Q-TOF LCMS-9030 Mass Spectrometer for my laboratory, and I would greatly appreciate hearing...

10 March 2024 9,081 0 View

Can I use this buffer made 1 years ago and storage a 4ºC?

The buffer contains: 100mM NaCI, 0.1% Triton X-100, 300mM Sucrose, 1mM MgC2, 1mM EGTA, 10mM PIPES (pH 6.8), and 100uM PMSF

23 January 2024 4,464 1 View

Gerrymandering or how to detect discriminative electoral rule change? it?

In public life, democratic mechanisms can be misused. Fitting electoral rules to desired results is called Gerrymandering to remember a prominent politician who was an expertin such Machiavellian...

03 January 2024 2,849 0 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Does anybody know why my chitosan/pva/gelatin hydrogel dissolve in PBS?

My final research is about making chitosan/pva hydrogel for wound healing using gelatin as crosslinker. Last month, i made the thin film hydrogel and it turn well. It was okay. Last week, i make...

28 July 2024 2,794 6 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

Daniel Oladapo Olaniregun

Aseptic packaging in sterile environment using inner material pack.

Yes if it will not cost me too much me. Let me first see the scale of what the researcher is handling

David Jensen

This publication may be of help:

https://www.researchgate.net/publication/260373640_Controlling_chitosan-based_encapsulation_for_protein_and_vaccine_delivery

Article Controlling chitosan-based encapsulation for protein and vac...