The following links may provide you some valuable informations:-

1. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3623776/

2. http://www.nepagene.jp/e_products_nepagene_0002.html

Integrating a 10kb gene by electroporation is very difficult, especially with this version of electroporator Biorad, without RF module.

It is very easy electroporate size of genes between 1 kb and 3 kb. Beyond 5kb is almost impossible.

For larger sizes of genes, it is preferable to use chemical agents such as PEI.

One note: for better electroporation efficiency, use a plasmid fresh (not frozen) to be supercoiled.

In contrast, for a chemical treatment, the more plasmid is linear (freezing and thawing) and the better the incorporation of the chemical reagent.

However, for a greater than 10 kD gene, it is more common to use a retroviral vector, such as a VLP associated with a cellular integration protein (fusion protein).

One last thing: the plasmid must be very pure (260nm / 280nm ratio = 2.0), it acts directly on the electroporation efficiency. Just as it is important: to use culture medium (serum free) to electroporate, to work at room temperature, and work quickly (no pre-incubation), for proper cell survival.

Finally 400V is the best solution, but only with all these remarks.