Thank you for your answer. What I mean with poor results is that when I make the slices the ant cuticle is almost always broken and the tissues are torn. In some slices the ant tissue separates of the rest of the slice (there is a hole in the place where the ant should be). I think it is because of the hardness of the cuticle. I also used a pepsin to get a softer cuticle, but it doesn't work very well. I use the usual procotol: the ants are frozen and then I embed them in tissue tek and make the slices.