Explain plasmid description?

Hello everyone, I got a plasmid containing E. coli, and its description on the tube is Bacterial stab (DH5a)_HlZ31_cr_Amp_, I know that Bacterial stab refer to LB medium, DH5a is the name of E....

14 January 2021 6,326 2 View

Can I do gene expression with partial cds (not the complete one)?

Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...

27 December 2020 3,688 3 View

How to calculate the Water Stress Index for a basin?

Water Stress Index (WSI) and Environmental water requirements (EWR). Thank you.

06 December 2020 904 3 View

What is a popular and standard model for quantifying land change drivers/determinants?

I'm looking for a standard model to quantify the land change drivers (e.g., forest to agriculture or vs). Actually, it depends on the data that I have and the specific location, but I would like...

22 November 2020 7,736 3 View

How can I discolor solution inside Ag/AgCl reference electrode?

Recently, I am using Ag/AgCl as RE in a basic three-electrode system to scan CV in K3Fe(CN)6/ K4Fe(CN)6. I realized that the color of the solution in RE changed from transparent to yellow. Can...

25 August 2020 6,254 7 View

I added Akaline Phosphatase and ligase buffer into my linearized plasmid without ligase but there are a lot of colonies after transformation.how come?

I am trying to find out what are the problems in my ligations. The first test: linearized Plasmid+ AP+ ligase buffer, after transformation, it gives a lot of colonies ! The second test:...

28 July 2019 8,550 3 View

How to combine fold change in microarray meta-analysis?

Hello, I would like to calculate the foldchange of differentially expressed genes after I meta-analyzed 9 datasets. I have calculated the fold change of those genes in each datasets but I wonder...

30 May 2019 459 3 View

Sample size for cluster analysis?

I have a follow-up question related to sample size for cluster analysis. Many recommend using 2^n - n is number of variables. I would like to ask if we should adjust for finite population...

30 January 2018 124 1 View

How could the morphology of anatase TiO2 (XRD) changed after grafting by amino propyl trimethoxy silane ? Thank you in advance.

I took XRD of TNTs and The modified TNT. And they were different?

14 February 2017 6,762 4 View

What is the effect of salt solution as the pore fluid on the pre-consolidation pressure of soils?

Dear all, I am doing oedometer test on remolded soils with sodium chloride (NaCl) as the pore fluid. The pre-consolidation pressure (Pc) was determined based on the method of Casagrande. The value...

13 December 2016 6,250 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

Can one use just 250 micron sieve & still get a good number of mycorrhizal spores from the soil?

In a situation where one cannot get the appropriate & separate levels of sieve for mycorrhizal survey work, can just one level of sieve give good results & spore counts?

13 February 2021 3,760 2 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View

Good day, I have not been able to amplify the plasmid Frt-hspB1. Somebody could give an advice me?

I acquired the plasmid in Addgene, reconstituted the filter paper where the plasmid comes from with 30 microliters of TE buffer pH 8, immediately afterwards perform the transformation of E.coli...

25 January 2021 2,526 3 View

Has someone used TTC agar overlay method and can help me?

I have tried to use TTC agar overlay method for a few times to identify respiratory-deficient yeast which were affected by EthBr. The problem is that cells which should have stayed white also have...

22 January 2021 585 10 View