Hi Kennedy,

you can use different antibodies to label the three different population in immunofluorescence:

TIG120 using anti SMA or Collagen Type1

HUVEC using anti von Wildebrand or CD31 (PECAM)

MCF10 or MDCK usian anti pan-Keratins

 Good luck

 Cesare

Hi Kennedy, 

I agree with Cesare comments, however be careful with aSMA because it mainly distinguishes myofibroblast (or differentiated fibroblasts). 

I strongly recommend you the use of PDGF Receptor alpha for fibroblasts or mesenchymal cells.

Best regards,

Thank you very much Cesare and Osvaldo. 

I will try out your suggestions and see how it goes.

I have tried Vimentin for TIG120 and VE-Cadherin for HUVECS but the result was not pretty.

I will report back. Thank you indeed.

Best regards