Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why there is no increase in virus titer after 24 hour of seeding for harvest?

I am working on virus production through bioreactor,my concern is regarding ,no increase in virus titer after 24 hour of seeding for harvest Usually we seed the fermenter and incubate for 72...

05 January 2021 3,860 2 View

Can anyone suggest how to remove H&E stain from the stained slide?

Dear friends i have only H&E stained slide and i have to do other special staining from this slide only. kindly suggest me the procedure for complete destaining of this slide.

13 December 2020 634 4 View

How many cells would be good to perform Chip seq for FoxP3 binding domain? Which fixative would be good for the same?

We are trying to find FoxP3 interactive domain in WT vs KO mouse regulatory T cells (Treg) through Chip Seq analysis. I am unable to get anything after pulldown although sonitation was good....

24 September 2020 244 4 View

How to identify fake research and predatory journal?

Fake researches and predatory journals

14 September 2020 4,530 7 View

Which professional society bodies in India related to Civil Engineering provide worthy periodicals for researchers/Engineers?

List out the best professional bodies which provide worthly and subject-related periodicals. Also, list out as per specializations like Structural Engineers, Geo-Technical Engineers, Environmental...

09 September 2020 1,151 11 View

Does anyone aware of a easy way to extract data on the number of publications per year on a particular research area?

Is there any easiest way? I need data of my research area for my upcoming review article.

12 August 2020 3,826 4 View

How to report ordinal regression ?

The method of analysis for my research is ordinal regression contains 6 ranked variables. But the goodness of shows unexpected and i am confused. Is this really possible, my data fit well or...

29 July 2020 2,291 3 View

Does web of science cover all PubMed indexed journals or both have different list of journal (May be some of same)?

24 July 2020 4,802 3 View

Why we did not use normal glass (silica glass)in the surface plasmon resonance(SPR) based sensor ??

I have designed a SPR chip by using normal glass slide(Blue star No. 4slide)/Cu(5nm)/Ag(50nm). But it shows very less depth in the reflectance curve at resonance angle. What should be the reason ??

13 February 2020 3,770 4 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View