How can I dissolve chitin azure (Sigma) to carry out chitinase assay?

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Cloudy/high background western blot - can anyone help me?

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Peter Elias Kidibule

There few papers talking about it here you have one of them

Sonia t.a

hope these articles will be useful.

Zainab Alwatheqi

thank you :)

Kurt Raisch

I would crash granules and gently stirr and eventually warm up to 45°C and it should dissolve within several hours. If still resisting, I would vortex. Otherwise I would filter the particles, weigh them dry and replenish them. In any case I would reclaim at Sigma.

David Kuida

Could you say what worked in the end? Zainab Alwatheqi