Can I use the CHARMM GUI input files for Gromacs in HPC?

Good day everyone! I am a Biochemistry undergraduate in the Philippines and to be very honest, we have no background in bioinformatics since we really focused on wet lab techniques + theory. Due...

15 February 2021 3,422 4 View

Cause of slow bromophenol blue migration and smearing while running SDS PAGE?

While running gels for western blotting, I am currently getting, what seems to be, the blue dye from my sample buffer migrating more slowly in sample lanes leaving blue streaks. These transfer to...

08 February 2021 5,317 3 View

Same sample, significantly different trace for DMF vs. THF for cyclic voltammetry?

Dear reader, I've been having trouble with my cyclic voltammetry recently, but I then had a break through and managed to get apparently "clean" results using dry, deoxygenated THF and/or DMF,...

06 January 2021 4,312 3 View

Can I use GROMACS for field-theoretic simulation of copolymers?

I need to know if I can use GROMACS instead of LAMMPS for my undergraduate thesis wherein I will generate phase diagrams of copolymers using FTS. Thank you so much!

22 November 2020 3,725 3 View

How to quantify lncRNA/miRNA in ribosomal depleted RNA-seq?

I did some ribosomally depleted RNA-seq in rat brain. Does anyone have recommendations on how to quantify/compare small RNA expression between samples? I have used RNA STAR to map reads back to...

11 November 2020 5,331 1 View

What are some essential steps when using THF for cyclic voltammetry?

I'm new to electrochem/cyclic voltammetry. I'm interested in measuring cyclic voltammograms of organic semiconductors in organic solvents, specifically, THF. I know that in principle I should be...

09 November 2020 2,298 4 View

At what stage of development do animals develop sentient?

I'm trying to find out in the development between being an embryo and an adult animal does the animal become phenomenally conscious? I'm especially looking for recommendations for articles on the...

30 August 2020 3,815 3 View

How to depolarize cultured primary neurons with KCl without them dying?

14 August 2020 1,488 8 View

Recommendations on model enveloped viruses that are easy and safe to propagate?

Hi everyone, We are synthesizing a virucidal material that will kill SARS-CoV-2 on contact. In these preliminary stages, we want to use enveloped surrogate viruses that are the easiest to...

08 August 2020 6,731 4 View

Where can I purchase fluorescent silica nanoparticles?

Hi all, I want to purchase fluorescent silica nanoparticles (with and without a functional group to make it hydrophobic) in powder form. A variety of size options would be key. Preferably, if you...

04 August 2020 7,440 6 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View