14 August 2017 1 8K Report

I've got a piece of lab equipment (a metal mesh) that has a ton of gel pieces stuck to it and on it. Is there some solvent or treatment that will remove the acrylamide?

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Is the sample size sufficient for using a fixed effects model?

For my research, I will retrieve data for each firm (100 firms) over 5 years, leading to 500 data points. Should this dataset size be sufficient for using a fixed effects model?

13 April 2024 6,669 2 View

Can freeze-drying remove 12-24% ethanol concentration from sample?

Hi, I have two samples containing 12-24% Ethanol (diluted by distilled water). I am wondering if these samples are frozen and then freeze dried, would that either decrease or get rid of the...

28 March 2024 5,961 1 View

I am looking for someone that can validate our Research Questionnaire about "Exploring Single-Use Plastics Towards The Environment In The Selected"?

I am currently in my senior year of high school and for our research, we have to find at least 1 expert around our topic to validate our questions. ps. title was cut off- "Exploring Single-Use...

26 March 2024 2,375 3 View

Separate IPA-water mixture?

Hi everyone, I am conducting an experiment which I am extracting substances into a IPA-water mixture. Since I am going to analyse the substances using GCMS, I need to somehow remove the water...

09 January 2024 4,060 3 View

PH of the rectum of cattle?

Hi all. I was wondering if anyone knew what the pH or pH range of specifically the rectum area in cattle? I've been looking for information on the subject but couldn't find any. Thanks!

19 November 2023 336 0 View

How do you curve fit an experimental S-N curve with the BS 7608 standard?

Hi, I have set of experimental data that I have collected from fatigue tests and I would like to ask how do I curve fit the data with the BS 7608 standard equation on excel? I have tried various...

05 November 2023 477 1 View

Where to find JavaScript version of Probabilistic Reversal Learning Task (reward learning)?

Does anybody know where I could find a freely available JavaScript version of the probabilistic reversal learning task (2 armed bandit; participants pick between 2 abstract stimuli w/different...

20 September 2023 8,842 0 View

How do you determine the Yield Strength Ratio to conduct a fatigue test?

Let's say I have the values of my material properties (e.g. UTS, Yield Strength, Young's modulus) after conducting a tensile test, and then I would proceed to conduct a fatigue test to investigate...

21 August 2023 608 2 View

Anion-exchange membrane?

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14 August 2023 9,706 1 View

How to find out the effect sizes that can be detected in a simple mediation analysis (given is the power, sample size and α)?

Hello, I am trying to find out which effect sizes can be detected with a simple mediation analysis, that was conducted in SPSS using the PROCESS Macro by Hayes (Model 4). I have the following...

07 June 2023 5,161 0 View

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Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

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What is the solvent for 8YSZ nano material?

I need to do some experiment with it in liquid form.

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Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

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How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

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Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

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I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to check pH of a high strength hydrogel?

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Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

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