How can I differentiate between Vascular smooth muscle cells (VSMC) and myofibroblasts in in vitro culture systems?

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Ali Burak Özkaya

Myofibroblasts originated from stellate cells usually express GFAP which is not expressed in VSMCs.

And as far as I know metavinculin and smoothelin are not expressed in myofibroblasts (but they are markers of VSMC).

Isehaq Al-Huseini

Thank you very much Ali Burak Özkaya

What about alpha-Actin?

aSMA is expressed in both Myofibroblasts and VSMCs. We use it as a marker for stellate cell activation.