I'm trying to develop a ratio of gene expression in a 3x2 design. Animals receive a certain treatment during embryogenesis, then later receive either a secondary treatment or vehicle. I need to compare "induction" between different embryonic exposure groups
Treatment Group
A B C
Drug
VEH
This would look as follows: ((A+Drug)/(A+VEH) vs. ((B+Drug)/(B+VEH))....
All gene expression values are normalized to a reference gene and log transformed. Normality and homoscedasticity assumptions are met for all groups.