I'm trying to develop a ratio of gene expression in a 3x2 design. Animals receive a certain treatment during embryogenesis, then later receive either a secondary treatment or vehicle. I need to compare "induction" between different embryonic exposure groups

                     Treatment Group

              A               B               C

Drug

VEH

This would look as follows: ((A+Drug)/(A+VEH) vs. ((B+Drug)/(B+VEH))....

All gene expression values are normalized to a reference gene and log transformed. Normality and homoscedasticity assumptions are met for all groups.

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