What is the difference between a mouse and a human complement system?

We have a human IgM antibody. It can induce cell death by CDC with rabbit or human complement serum. I am wondering whether mouse complement serum will also work?

08 September 2014 1,517 12 View

Does anyone know why a hybridoma culture turn red and not yellow?

I have a IgG secreting hybridoma. Unexpectly, when the cell number increase, the culture turn red and the cells are not happy. Any suggestions?

08 September 2014 5,563 10 View

How do I identify the target of cell membrane binding IgM?

08 September 2014 9,495 2 View

Can anyone help with the conversion of IgM hybridoma to IgG secreting one?

We have an IgM secreting hybridoma and want to convert it to a IgG. Any suggestions? Thanks a lot.

08 September 2014 2,014 11 View

Does anyone know why starvation can enhance the antibodies intake of cells?

Cancer Biology & Therapy 7:5, 752-759; Monoclonal antibodies target intracellular PRL phosphatases to inhibit cancer metastases in mice. In the above paper, the authors noted that stavation...

08 September 2014 539 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

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28 February 2021 9,936 2 View