How can I determine whether the antigen of an antibody is a protein?

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What is the difference between a mouse and a human complement system?

We have a human IgM antibody. It can induce cell death by CDC with rabbit or human complement serum. I am wondering whether mouse complement serum will also work?

08 September 2014 1,665 12 View

Does anyone know why a hybridoma culture turn red and not yellow?

I have a IgG secreting hybridoma. Unexpectly, when the cell number increase, the culture turn red and the cells are not happy. Any suggestions?

08 September 2014 5,637 10 View

How do I identify the target of cell membrane binding IgM?

We identify an IgM antibody from human, which specifically binds to the outer membrane of breast cancer cell line MDA-MB-231 and is good for CDC in this cell line.Is anyone thinks it is...

08 September 2014 9,568 2 View

Can anyone help with the conversion of IgM hybridoma to IgG secreting one?

We have an IgM secreting hybridoma and want to convert it to a IgG. Any suggestions? Thanks a lot.

08 September 2014 2,093 11 View

Does anyone know why starvation can enhance the antibodies intake of cells?

Cancer Biology & Therapy 7:5, 752-759; Monoclonal antibodies target intracellular PRL phosphatases to inhibit cancer metastases in mice. In the above paper, the authors noted that stavation...

08 September 2014 616 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View