Huiwu Zhao

6 Questions 14 Answers 0 Followers

Questions related from Huiwu Zhao

How can I determine whether the antigen of an antibody is a protein?

We have some antibodies which can stain the cell membrane. What kind of experiments can I do to make sure the epitopes they recognize are from proteins? Thanks a lot.

10 October 2014 359 3 View

Does anyone know why a hybridoma culture turn red and not yellow?

I have a IgG secreting hybridoma. Unexpectly, when the cell number increase, the culture turn red and the cells are not happy. Any suggestions?

09 September 2014 5,614 10 View

What is the difference between a mouse and a human complement system?

We have a human IgM antibody. It can induce cell death by CDC with rabbit or human complement serum. I am wondering whether mouse complement serum will also work?

09 September 2014 1,623 12 View

How do I identify the target of cell membrane binding IgM?

We identify an IgM antibody from human, which specifically binds to the outer membrane of breast cancer cell line MDA-MB-231 and is good for CDC in this cell line.Is anyone thinks it is...

09 September 2014 9,542 2 View

Can anyone help with the conversion of IgM hybridoma to IgG secreting one?

We have an IgM secreting hybridoma and want to convert it to a IgG. Any suggestions? Thanks a lot.

09 September 2014 2,072 11 View

Does anyone know why starvation can enhance the antibodies intake of cells?

Cancer Biology & Therapy 7:5, 752-759; Monoclonal antibodies target intracellular PRL phosphatases to inhibit cancer metastases in mice. In the above paper, the authors noted that stavation...

09 September 2014 596 1 View