I got samples from chicken gizzards fed with different oyster mushroom, and streak inoculated them on PDA plate after 10-6 serial dilution, now how can I determine the bacterial colony forming unit?
Right.. so you serially diluted 1/10 6 times. But what is the volume you used to streak the plate? Usually it's 100uL, unless you used a 10uL or 1uL loop. What volume you used to plate for your colonies needs to be accounted for since 3 colonies from 10uL is 3cfu/10uL, which is different than 3cfu/100uL. And then you account for the serial dilution factor.