Transcriptome fungal gene expression
I got samples from chicken gizzards fed with different oyster mushroom, and streak inoculated them on PDA plate after 10-6 serial dilution, now how can I determine the bacterial colony forming unit?
02 March 2020 4,823 3 View
I have seen many questions on papers rejected after for many months of delay.. it could be very frustrating and devalue the paper if it is time sensitive. I know it depends of the reviewers...
01 February 2020 3,963 2 View
Does it really matter?
31 December 2019 5,835 4 View
Often time I see scientists using both forward and reverse primers for amplification of a particular gene.. Is it really necessary??
31 December 2019 3,804 6 View
08 September 2017 5,781 0 View
Molecular Biology
08 September 2017 8,921 0 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...
01 March 2021 1,133 1 View
Incompletely cross-linked pdms are difficult to remove.
01 March 2021 798 3 View
Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.
27 February 2021 9,356 3 View
Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...
26 February 2021 4,026 5 View
What is the most suitable medium of ascomycetes fungi ??
26 February 2021 4,413 1 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
The main studies are as follows: * regular and timely receipt of information about the state of the environment using automated systems (stationary and mobile); * operational analysis of the...
23 February 2021 912 5 View
I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...
16 February 2021 169 4 View
It has been established that quarry mining site polluted and contaminated it's environment with heavy metals, these affects the crops and vegetables planted in the area. The possible solutions to...
12 February 2021 1,155 5 View