you can go for RNA isolation from the inactivated vaccine. there is high probability that the obtained RNA can be used as +control if the primers get sufficient itact fragments to bind in realtime pcr amplification. I had done it once and it served my purpose.

Hi,

Yes, you could use a vaccine as a positive sample in a PCR run, but it would not be a true positive control of the clinical material that you are testing. You would need a tissue or a body fluid containing the nucleic acid that you are detecting, instead of an isolated RNA. This clinical material should then be subjected to the whole process including extraction and amplification.

Apart from this methodological issue, please consider all the substances that are added to the vaccine during the manufacturing process. If you are talking about inactivated vaccines, then you will easily get false negative results due to the  (potential) inactivation of the polymerase caused by the oil or any other component of the vaccine. If you can get a modified live vaccine containing no oil or other excipients, then the chance of detection increases.

In our experiencia both the DNA and the RNA are denature during vaccine manufacturing. But the most limiting factor is not the integrity of them but the presence of inhibitors

Best regards

I  did RT-PCR for dog for Rbies virus . the dog is clinically normal , but I got Positive result (900 bp pcr band) . Do you think it is false positive do to vaccination. ( the vaccine is inactivated RNA virus  vaccine).  I did negative control and it is OK

The dog is clinicaly normal how time ( days). The sample is from what? saliva, lacryma....

can you use simultaneous :

The sample and 

the vaccine ( the same batch used in vaccination )  Intracerebrally  ( 2 or 3 dilution ) on suckling mice.

Please go for sequencing and confirm the amplification is related to vaccine strain or not. You need to take proper sampling, because inactivated vaccine mostly present in lymphoid organs or lymphoid aggregates for longer time.