I want to check eosinophil level in the mice blood/serum, for my experiment, using ELISA kit. need your advice, Thank you !
In human we have shown that plasma EDN levels detected with an ELISA correlate very well with blood eosinophils numbers. EDN levels are well measurable in human plasma samples. Alternative surrogates are IL5 or IL13 but those levels are very low in concentration and you need high-sensitivity technology to reliably quantify those cytokines.
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Daniel S. Strasser Thank you so much for your answer, I want to check it in mice samples, serum and BAL fluid. Is there any specific difference I should consider for mice serum and BAL compared to human ?
also, how appropriate would it be to measure Eosinophil peroxidase or eosinophil proteoglycan2 (PRG2) also known as major basic protein(MBP).
I would really appreciate your response/advice.
Thank you very much !
Several ELISA kits are available in scientific markets for detecting eosiniphil levels.But it is better to check it by using radial diffusing method that is less costy and no long time consume. thanks.
We have used EDN for serum and sputum samples and it should work in BAL as well. And I would assume other eosinophil marker such as EPX, ECP or MBP should work as well. In human tissue staining of eosinophils EPX works very well (see Article Eosinophilic Esophagitis: Relevance of Mast Cell Infiltration
)MPO activity is frequently used as surrogate for neutrophils. I'm not aware of a similar assay for EPX.
Having a quick look I would chose for EPX ELISA because companies of known quality provide such ELISAs e.g. https://www.lsbio.com/elisakits/mouse-eosinophil-peroxidase-epx-elisa-kit-sandwich-elisa-ls-f7707/7707?trid=247.