Hello, if you have sequence of your gene of interest you can easily design primer by any of primer design program.
Hello, if you have sequence of your gene of interest you can easily design primer by any of primer design program.
You may look at a multi-sequence alignment of the orthologous genes in related species. Regions that are highly conserved in this alignment are very likely also conserved in the species you are interested in, and primers directed against these regions therefore likely to also recognize the gene in your species of interest.
Ahmed, that will depend on the kind of data that you DO have and the kind of information you want to obtain.
If you already have the sequence you are after, things are simple: you just use it to design the desired primers. If you know the gene you are after, have no sequence available for it but there are related species with available sequences, follow the suggestions given by Annemarie.
If you do not have the sequence and you do not know/have orthologous sequences, the path will be longer and my suggestion would be that you speak to a geneticist or bioinformatician about your specific needs. They will be able to give you a much more specific answer.
If you have mapping populations available, you might need to actually map your gene. If you have access to NGS technology, you could do a quick (10x coverage for example) sequence of you genome or transcriptome and look for the sequence of interest (however, if you have no annotated sequenced genome, you may need help).
Good luck with your experiments!
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