Hi there, I'm looking for help to deposit RNA from DEPC-treated water.
I used Trizol to do the total RNA isolation and finally dissolve the RNA in DEPC-treated water. However, I found I did a wrong calculation about the volume of the DEPC-treated water, which cause the low concentration of RNA. How can I make the RNA be precipitated from the DEPC-treated water? It would be pretty kind if someone can help me out.
Plus, can I just follow the protocol from adding isopropanol to deposit RNA?
You can re-concentrate the RNA by letting the water evaporate. Open the lids to your tube and let them sit overnight in the freezer
Katie A Burnette Thanks for your help. I'm a little worried about the RNase contamination and the following RNA degradation if I open the lips. I'm considering whether I can add isopropanol in the DEPC-treated water just like depositing RNA from the aqueous phase after extraction with Trizol and chloroform.
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