Vitamin D receptor immunohistochemistry in the brain?

I'm struggling to detect VDR consistently using immunohistochemistry in the mouse brain. Does anyone have a protocol or antibody suggestion? I'm also having difficulty finding other papers that...

03 April 2019 2,267 1 View

Guinea pig spinal cord anatomy?

For the first time I am working with guinea pigs and need to report on the spinal cord. Can anyone suggest resources I can use to accurately describe guinea pig spinal cord anatomy? Is guinea pig...

01 February 2016 872 3 View

Tips for dissecting the submucosal plexus in the mouse GI tract?

Does anyone have any tips on how to dissect a whole mount mouse submucosal plexus? I am currently doing it by taking off the muscosa, flipping the tissue, then removing the longitudinal and...

01 February 2015 6,344 1 View

Do HEK293 cells express IL-10 receptor?

06 July 2014 4,855 2 View

What is a quick way to measure gut microbiota?

06 July 2014 4,975 17 View

Does anyone have an opinion on the best method to examine neurotransmission to smooth muscle in the enteric nervous system?

I'm studying the effects of SMN on smooth muscle in the gut, and because the enteric nervous system lacks NMJs but rather has neuronal varicosities that secrete neurotransmitters, I'm wondering if...

01 February 2014 6,128 6 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View