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Questions related from Sara Gombash Lampe
I'm struggling to detect VDR consistently using immunohistochemistry in the mouse brain. Does anyone have a protocol or antibody suggestion? I'm also having difficulty finding other papers that...
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For the first time I am working with guinea pigs and need to report on the spinal cord. Can anyone suggest resources I can use to accurately describe guinea pig spinal cord anatomy? Is guinea pig...
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Does anyone have any tips on how to dissect a whole mount mouse submucosal plexus? I am currently doing it by taking off the muscosa, flipping the tissue, then removing the longitudinal and...
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What is the best way to overlay tracings from a GI tension organ bath assay and calculate and average or show representative tracings? I'm working in LabChart, but I'm not having any success. Thanks.
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Can anyone tell me if HEK293 cells naturally express the IL10 receptor (either subunit)? Also, has anyone tried a transfection of 293's with a viral vector expressing IL10R?
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I'm looking for a method to collect preliminary information on changes in the gut microbiome in mice. 16s rRNA sequencing would be the ultimate goal, but I'm looking for a way to determine if...
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I'm studying the effects of SMN on smooth muscle in the gut, and because the enteric nervous system lacks NMJs but rather has neuronal varicosities that secrete neurotransmitters, I'm wondering if...
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