Vitamin D receptor immunohistochemistry in the brain?

I'm struggling to detect VDR consistently using immunohistochemistry in the mouse brain. Does anyone have a protocol or antibody suggestion? I'm also having difficulty finding other papers that...

03 April 2019 2,267 1 View

Tips for dissecting the submucosal plexus in the mouse GI tract?

Does anyone have any tips on how to dissect a whole mount mouse submucosal plexus? I am currently doing it by taking off the muscosa, flipping the tissue, then removing the longitudinal and...

01 February 2015 6,344 1 View

How can I create a tracing overlay image for GI tension assay?

What is the best way to overlay tracings from a GI tension organ bath assay and calculate and average or show representative tracings? I'm working in LabChart, but I'm not having any success. Thanks.

07 August 2014 1,791 1 View

Do HEK293 cells express IL-10 receptor?

06 July 2014 4,855 2 View

What is a quick way to measure gut microbiota?

06 July 2014 4,975 17 View

Does anyone have an opinion on the best method to examine neurotransmission to smooth muscle in the enteric nervous system?

I'm studying the effects of SMN on smooth muscle in the gut, and because the enteric nervous system lacks NMJs but rather has neuronal varicosities that secrete neurotransmitters, I'm wondering if...

01 February 2014 6,128 6 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

How can we calculate the sample size for rodents for a particular period of time?

How can we calculate the sample size for rodents for a study for one year? Are there any specific protocols to be followed for designing rodent sampling? How long should be the sampling interval...

01 March 2021 1,056 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

Do you have papers on hindlimbs myology of birds to share?

Hi all! My work team and I are looking for papers on muscular descriptions of the hindlimbs of birds (extant and extinct) that include the intrinsic muscles of digits. Any help will be more than...

22 February 2021 8,330 6 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View