BSEM/SEM approach?

Hi all! I'm getting into BSEM approach and I am specially concerned about when it is recommended or not to use this model. I have tested a BSEM with a small sample obtaining excellent results,...

07 September 2020 3,919 3 View

Can you help me to find and share material by Ernesto Spinelli?

I am an Italian psychologist, currently I am a professor at the University of Sherbrooke, I work in the field of the existential humanist approach, especially the role of the art in palliative...

23 May 2020 6,863 3 View

Is there an easy way to derivatize silsesquioxanes?

I have a polymer end-capped with a Polysilsesquioxane (POSS) like the one here: https://en.wikipedia.org/wiki/Silsesquioxane#/media/File:Silsesquioxane_T8_Cube.png where *R=i-butyl*. I haven't...

12 March 2020 2,590 3 View

What solvent mixture/ratio should I use for preparing liposomes by REV method?

I have tried with chloroform-methanol (2:1 v/v) but the emulsion produced after sonication is not stable. Also, lipids don't dissolve well in pure ethyl ether.

24 February 2020 2,360 5 View

Could you suggest any scale to assess parents' satisfaction with their children's schools?

Hi all! I'a looking for a scale to assess parents' satisfaction with their children's schools. I have found some different papers about parents satisfaction but no one seems to offer just what I...

10 December 2018 8,430 3 View

Loading control for WB in High Five insect cells?

30 August 2017 7,901 6 View

Can I extract mRNA from fixed cells?

I want to sort my cells using a marker that require intracellular staining and so, fixation and permeabilation. After that staining, and sorting, can I use these cells as source for mRNA extraction?

01 November 2016 1,286 1 View

No title

30 May 2016 4,100 10 View

What is the best programming language for an open-source computer vision project? Which one would you prefer as potential developer?

We have recently developed a tracking system capable of following several individuals keeping their correct identities in spite of crossings or overlaps (www.idtracker.es). It will be published in...

13 May 2014 9,685 72 View

Is there an efficient algorithm to compute the FFT of sparse binary data?

I have to compute the FFT of sparse binary images - where pixel values are either zero or one, but mostly zero. I'm currently using FFTW under MinGW but I'd like to know if a faster implementation...

24 September 2012 9,981 9 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View