For the purpose of my research, I am interested in representing the results of two enzymatic activities and would like to represent them in embassies in (umol/ml/min).
Hi there,
1 Unit is the amount of enzyme which in the assay allows the formation of 1µmol/min of product. So the only thing you have to do is to divide the number of unit per 1000 (1mL is 1/1000 of one litre).
What Dominique Liger wrote is valid, if we're talking about the International unit
https://en.wikipedia.org/wiki/Enzyme_unit
But enzymes often have their own defined units, so you first have to find out, how is your unit defined.
It depends on the nature/ type of substrate being acted upon by the enzyme and the way you define an enzyme unit (U). Usually, one U of an enzyme is it's amount that converts one micromole of it's substrate into product. Which enzyme are u using?
Shamsher S. Kanwar I am measuring the activity of the LDH enzyme, detecting the decrease in absorbance at 340 nm (NADH Absorbance).
U/L=(micro mole x 1000)/(aliquot added to well in ml x reaction time)
you can check this link also
https://www.aacc.org/~/media/files/meetings-and-events/resources-from-past-events/conferences/2015/profprac/slides-and-handouts/laboratory_calculations_handouts_jones.pdf?la=en
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