Distinguishing between human activated and exhausted lymphocytes T?

Any suggestions on how to distinguish between activated and exhausted human lymphocytes T CD8 with flow cytometry? It is known that exhausted lymphocytes T have higher expression of inhibitory...

28 December 2020 255 3 View

Is this conference a scum?

Hi, can anyone help me to understand if this conference about phytoremediation is a scum? https://waset.org/phytoremediation-phytotechnologies-and-management-conference-in-july-2020-in-prague...

15 January 2020 8,841 3 View

Help with lentiviral vector?

Hi everyone, I am trying to produce eGFP lentiviral vector by transducting HEK 293T with calcium phosphate method. I transduce cells when they are at 70 % confluent (following this method...

14 October 2018 2,351 4 View

Cross-sex genetic correlation

Hi! We are trying to estimate body mass (W) heritability and cross-sex genetic correlation using MCMCglmm. Our data matrix consists of three columns: ID, sex, and W. Body mass data is NOT...

25 July 2017 6,702 1 View

Which are the best melanoma cell lines?

Hello, I'm working on melanoma and I'm searching the best melanoma cell lines (easy to culture) recapitulating all the steps of melanoma growth: Radial growth-phase cell line, vertical...

05 June 2017 8,061 2 View

I would like to extracte cell membrane from frozen buffy coat. Can anyone suggest a reliable protocol?

Hello everyone, I need to isolate cell membrane of leukocytes and platelet, from frozen buffy coat. Any suggestion?

20 September 2016 5,749 1 View

Fibronectin antibody clone FBN11?

I wish to stain fibronectin in 3D tumor spheroids made with human cancer cells and fibroblasts. do you have a good antibody to suggest? Have you ever used the references MA5-11981 from...

30 June 2016 1,973 2 View

Does the rule of choosing a component if the model performance increases by >5% measured by RMSEP (in cross-validation) apply also to PLS technique?

When choosing a WAPLS model, apart from relying on performance (e.g. r2, r, max bias, RMSEP, scatter-plot etc..), the recommendation is to select the successive component if the model performance...

15 May 2016 1,200 2 View

How can I effectively debenzylate a tertiary amine?

31 March 2016 9,050 8 View

Multiple choice feeding experiments - Can anyone help me decide on my statistical approach?

I performed a multiple choice experiment to assess the food preference of the sea urchin Paracentrotus lividus in relation to 5 different food items. I have 15 replicates, each represented by a...

03 February 2016 8,060 4 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View