I am working with using gold nanoparticles to find sequence specific proteins. I attached the thiolated sequence specific oligo (sequence specific to one DNA binding protein) to gold nanoparticle for control. I transfect the nanoparticle inside the cell but I am getting lot of noise. I am getting many non specific protein attached to my nanoparticle only. I did the MS. It has 135 proteins including albumin of serum media. I heard of innovacoat of innova biosciences in which there coating material will not react to any protein inside the cell and proteins will bind to my oligo. Is there anything like that or any protocol of coating nanoaprticle? I am confused. Help please.
Thank you sir for your pointers.That would definitely lead me to right direction. I will read and try to modify and do experiment as per your suggestions.Will get back to you for sure. :)