How can I clone and express 3Kbp of DNA?

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SLN

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Which are the best ways to analyze the data of PM2.5 & PM10 in the atmosphere collected by gravimetric analysis?

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Mass scaling in ABAQUS ?

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How to model a resistor whose resistance can be varied w.r.t time or temperature in MTALAB, kindly help?or do i get a ready element with such feature?

MATLAB,

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How do i represent electric ARC in MATLAB and measure its parameters like voltage, current and temperature, kindly help me with the circuit?

MATLAB simulink

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When crystal field splitting does not occur even in the presence of ligand?

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Currently, i am working on DFT modelling in BURAI 1.3 (Quantum espresso). While i am running my simulation, error was appeared as shown below image?

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How we find purity of extracted cellulose using FTIR analysis?

What is the method for determination of cellulose purity using FTIR analysis which is extracted by waste biomass?

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What is the method for determination of purity of cellulose extracted from biomass?

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Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

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How to confirm the site-directed mutagenesis result without performing NGS?

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08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Does crude extraction using NaOH and Tris work well with Fungi?

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Can I use a HisTRAP column for affinity chromatography?

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Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

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Weak DAPI staining after immunohistochemistry - how to improve?

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Where should the ARS sequence be introduced on a plasmid?

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

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Toshisangba Longkumer

What actually is your problem? Is it that you can't insert in a particular vector or you don't know which vector to use?

Ganesh Shastry

I have to express a viral gene which is of 3Kbp in E.coli I don't know which plasmid to use because the I have never worked with such a big insert