How can I clone 3Kbp of insert into a vector and to express the protein in E coli system?
What actually is your problem? Is it that you can't insert in a particular vector or you don't know which vector to use?
I have to express a viral gene which is of 3Kbp in E.coli I don't know which plasmid to use because the I have never worked with such a big insert
We are doing a study on impact of aerosol on precipitation. Which satellite data and which wavelength will be the best. or can some one suggest a criteria to be adopted to choose an instrument...
12 February 2021 6,324 3 View
As per the rules and regulation DOI is needed and paper has to be opened in online by the anna university rules let me know whether the published article in some journal and the DOI issued by...
27 November 2020 7,540 2 View
commonly used practices for the identification and characterization of the kind of cell in cord blood other than Flowcytometry and reverse-transcription polymerase chain reaction (RT-PCR).
08 August 2020 6,881 3 View
Though both the methods look similar I believe there may be differences in them. What are the disadvantages of cut-cell meshing method and why is not widely used in research in comparison with...
12 July 2020 5,636 3 View
I tried to contact [email protected] but no response so kindly help me in this regard. We have an Advantec GS-990, it was purchased a long time ago from a Korean Collaboration. Currently,...
16 June 2020 9,890 1 View
I have done Kinetics study of two different probes namely Fe(CN)63-/4- and Ru(boy)2+ on glassy carbon electrode. I kept the potential between -0.2 to 0.6 and 0.8 to 1.2 respectively. In FeCN when...
25 April 2020 3,364 10 View
On what scale can SIMSCALE simulation software be helpful for students in their engineering course work comprising CFD ( Computational fluid dynamics ), thermal and structural concepts? Can...
29 March 2020 2,952 3 View
I came across the RIR value of Martensite in a paper, which is 7.98. Similarly for Austenite, which is 10.81. I want to quantify Retained Austenite in my heat treated samples. I am using Reference...
26 February 2020 9,401 2 View
I have calculated electronic components of thermal conductivity by wiedmanfranz law. Then the lattice thermal conductivity is derived from it. But some of those values are negative. at the end of...
26 January 2020 1,507 4 View
Hi friends, I have been analyzing a protein family, called Fibrinogen domain related proteins, commonly called FReD or FREPs. I confronted with problem that C terminal part of this family proteins...
08 January 2020 1,320 1 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View