How can I choose an appropriate statistical model for the sample size calculation?

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Has anyone detected immunoprecipitated endogenous protein by Coomasie or Silver Stain?

I am trying to detect an endogenous protein of around 135KDa by Coomassie or Silver Stain for a downstream analysis. My immunoprecipitation works well, and the protein seems highly enriched in...

12 January 2023 7,528 4 View

Does anyone have human primer sequences for making 28S ribosomal RNA probes for ISH?

I am trying to establish a control probe for RNA in situ hybridization for human samples. The plan is to synthesize DIG-labeled probes by in vitro transcription. Does anyone have primer sequences...

17 July 2022 5,324 0 View

Can you provide me some of the major writings about the "Stranded Biharis in Dhaka"?

This is needed for my reasearch work.

10 May 2022 6,895 2 View

Can the host of IP antibody and the WB primary antibody be same?

If I am doing an IP for a mouse and a human protein with an antibody that is raised in Rabbit and recognizes both the mouse and human protein, and later when I want to do WB, is it okay if the...

13 December 2021 5,516 3 View

Can biasing change the work function of same metal in different bias condition (forward and reverse)? What is the fact behind of this change?

In metal-semiconductor-metal junction, biasing may tune the band bending at the interface of metal and semiconductor. How does this biasing affect the work function of forward and reverse biased...

22 March 2021 978 2 View

What is an acceptable FDR% for proteomics data publication?

Is there an established convention on this? I have heard from experts that 1% FDR is recommended. I was wondering can we report protein IDs at 5% FDR. Is there any published guidelines on this? If...

25 August 2020 1,556 4 View

Is there any way to calculate minimum free energy of RNA molecules which are more than 10000 bases in length?

The two popular software UNAfold and RNAfold server cannot go beyond 10,000 nt while calculating minimum free energy (MFE) of RNA molecules. Is there any way around to tackle this issue?

29 March 2020 4,350 3 View

Any suggestions on high quality sequencing service providers in India?

Can anyone suggest some service providers in India who have good reputation of providing better sequencing service and also have reasonable charges?

24 January 2020 1,829 0 View

Does anyone have a good protocol for dissecting the second order mouse pulmonary artery?

Does anyone have a good protocol for dissecting the second order branch of the pulmonary artery from mouse. I am interested in performing some pressure myography studies.

18 December 2019 9,204 1 View

How to calculate Reynold's number for rheology of low viscous fluid in coaxial geometry (steady-state rheology) ?

I have varied the shear rate from 1s-1 to 500 s-1 and wish to study the change in Reynold's number for a juice with 18% solid concentration. How to calculate the velocity term? or can it be...

16 October 2019 2,093 5 View

What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,629 1 View

What may be the reasons for failures of Tube toi Tube Sheet Joints in Boiler Drum ?

We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.

08 August 2024 3,161 0 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

How to calculate effect size of AMCE (Average Marginal Component Effect) in Randomized Conjoint Experiment?

I am following Hainmueller, Hopkins, and Yamamoto's (2014) paper for my randomized conjoint experimental data analysis. The link to the paper is provided below. I received a comment from the...

02 August 2024 4,406 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

How to conduct a sensitivity power analysis for Kendall's Tau?

Is there a straightforward way to conduct a sensitivity power analysis for a Kendall's Tau correlation? I was considering using the sensitivity setting and "Correlation: point biserial model" test...

28 July 2024 6,133 8 View

How to estimate sample size for GWAS of continuous and discrete traits? What are the pre-requisites?

Genome-wide association study (GWAS) Continuous traits: eg. Height Discrete traits: eg. Eye color

28 July 2024 286 0 View

Reason for discontinuities in my Band structure?

Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...

25 July 2024 2,920 11 View

What is the best method for removing paraffin from plant samples prepared for microtome?

...

24 July 2024 3,087 3 View

Is it fare for editors of reputed Journals to put on hold manuscripts more than six months without review updates, Are they harassing researchers?

It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...

24 July 2024 8,596 2 View