19 December 2019 1 9K Report

Does anyone have a good protocol for dissecting the second order branch of the pulmonary artery from mouse. I am interested in performing some pressure myography studies.

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How do I compute alternatives of the Fuzzy Analytic Hierarchy if there are ALOT of them?

I have gotten the average grades of 25 students (They are both Anonymous). These 25 are my alternatives. Criteria from C1............CN are subjects and extracurricular activities. If in one...

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What is an acceptable FDR% for proteomics data publication?

Is there an established convention on this? I have heard from experts that 1% FDR is recommended. I was wondering can we report protein IDs at 5% FDR. Is there any published guidelines on this? If...

25 August 2020 1,231 4 View

What if People have a VERY different judgment in FAHP?

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07 July 2020 7,698 1 View

Appearance of crossing point signal even in water during qPCR. Help?

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01 March 2020 1,408 3 View

Which fungi species can be employed to hydrolyze insect protein?

I want to perform a solid state fermentation of freeze dried grasshopper and I'd like to know which fungi can be used to hydrolyze the protein from this insect. Thanks in advance!!

27 February 2020 4,638 3 View

Should non-renewable natural resources be exploited in indigenous territories? yes, no; why?

Consider that the communities disagree, and the government requires economic resources for works and projects (health, housing, roads...).

11 December 2019 4,159 16 View

Should non-renewable natural resources be exploited in indigenous territories? yes, no; why?

Consider that the communities disagree, and the government requires economic resources for works and projects (health, housing, roads...).

01 December 2019 10,634 16 View

How can I ask for permission to reproduce a figure from ThermoFisher.com?

I am currently about to finish my doctoral thesis (hurray!) just to find out that I forgot to ask for permission to some of the figures I used to illustrate it, mostly schemes for the...

28 November 2019 6,104 1 View

Does anyone have any recommendation for a good kit for total RNA isolation from small sample size?

I am looking for a kit that can yield good quality RNA from small sample size (around 10 000 - 15 000 cells). I have tried with Trizol, but so far have been unsuccessful in getting good enough RNA...

22 May 2019 1,998 7 View

Protein misfolding behaviour?

Hi all, I am working on a tricky protein. I surpassed some initial aggregation behaviour by adding 0.3% CHAPS and 1mM TCEP, buffer in which is happy until the removal of the SUMO tag, then it...

09 December 2018 9,460 3 View

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