the embryo belongs to which species? Is the seed a monocotyledon or dicotyledon?
if you mean germination in MS medium; in generally embryo growth begins by swelling, then the elongation of the radicle, followed by the appearance of small sheets of clear green color; which becomes darker with time.
You can record visual observation, or you can record weight after taking out from medium aseptically
First you should imbibe the seeds in distilled water for hrs. and then check it for the imbibation of seeds.. It also depends on the type of seeds u used for the germination whether it is monocot or dicot.. then you put it on MS media for germination and checked it for % germination...
Tthe embryo of di-cotyledons seeds generally have a heart-shaped structure, embryo growth goes through these steps:(according to the steps that I have observed during my work in the lab)
-significant swelling in the apical part (volume increase in length and in width)
-appearance of a small structure at the upper side, which is the upper apical meristem (caulinaire)
-This meristem will give the leaf primordia and elongation of embryo and formation of a small radicle
sometimes there is apparition of embryos on the first embryo it's depend on the MS medium
I don't know for papers, but I found this, maybe it can help you
Good luck
http://rdo.psu.ac.th/sjstweb/journal/27-Suppl-3/01_carbon_sources.pdf
http://www.sbmp.org.br/cbab/siscbab/uploads/b187eea6-a7f0-d880.pdf
http://www.scielo.br/scielo.php?pid=S1984-70332012000300002&script=sci_arttext
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000100014&lng=en&nrm=iso&ignore=.html
Dear Massakib Bekkaye Thanks for Your Kindness,
Best Regards,
Morteza
I don't have enough detail about your system. BUT, if you are testing dormant seeds, you definitely do not want to incubate them on any sort of MS medium. If you look at the chemical composition of MS, you will find many substances that can act as seed dormancy-breaking triggers alone or in combination with other stimuli...
Hi Dear Dr Marc Alan Cohn,
Thank you very much, In your opinion what are the best conditions for embryo dormancy studying?
need a genus & species; when were the seeds harvested etc. Experimental conditions will depend on the problem and the question the experiment is trying to answer.
yes of course, the embryo harvested from two location in iran (temperate and subtropical zone) and that is tree seeds in rosaceae family, but what is the best medium for embryo dormancy studying? too how checked them growth?
Generally dormancy results from a balance between growth hormones: abscissic acid and gibberellin, and also there are several types of dormancy embryo; integumentary, and mixed
in our lab we work with gibberellin who induces germination, and the seeds are placed in a temperature of 4 °C for (X) time to break dormancy
X depends on the species
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany
- Plant Biology