May I know how to run BAPS software for genetic structure analysis?

I converted my genepop file into BAPS file using CREATE. I have read the manual yet unable to understand how to proceed further. Any recommendations would be helpful.

20 April 2020 6,166 1 View

How many mouse splenocytes were stored in each cryovials?

I am planning to cryopreserved mice splenocytes in 10% DMSO +90% FBS media ( 1ml is added in each vial) for future experiments. I am NOT sure what's minimum number of mice splenocytes cells should...

03 December 2018 5,771 1 View

What tests can be used for descriptive research?

Hi, will appreciate expert opinion on the below: 1. Can 'impacting' or 'relationship' hypothesis be formulated for descriptive research? 2. Can correlation, regression and chi-square tests be...

27 March 2018 4,858 7 View

Can we perform Flow cytometry based NK cell cytotoxicity assay using melanoma cell line (B16F10)?

I did Flow Cytometry based NK cell cytotoxicity assay using anti-CD71 targets K562 and U266 cell line. Now I want to repeat same experiment using melanoma cell line (B16F10). Would you please...

06 December 2017 1,724 3 View

define Cloud Computing and Data Masking Techniques?

hi anyone working on data masking techniques?

27 November 2017 4,103 3 View

In WSN,is it necessary for nodes to receive all packets broadcasted by a node lies in their periphery?

19 October 2016 2,534 4 View

Why the saturated magnetization decreases when iron nanoparticle is coated with Chitosan and increases when coated with CTAB?

14 October 2016 3,647 1 View

Which algorithm is better than hough transform for edge detection?

edge detection algorithm in image processing

29 February 2016 729 8 View

Can anyone suggest how to calculate the concentration of the compound through peak area and RT using GCMS for FAME?

27 March 2015 8,137 10 View

Nsi I enzyme

Does any body had used Nsi I enzyme from MBI- Fermentas, i have used three different lot of the enzymes and in my case this enzyme is not working. I have checked it with three different plasmids also.

11 May 2011 1,440 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

No title

03 March 2021 5,360 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View