Hello everyone,
I would like to know which would be the virus multiplicity of infection (MOI) to infect, for example, an ex-vivo retina or an eye-cup derived from induced pluripotent stem cells, where I do not know how many cells are there.
I only have read that for infecting cell lines (in which you can count how many cells you have) you can use between 5x104 and 1x105 viruses per cell.
Could you please help me with this?
Thank you so much in advance.
Hasan Issa
It is going to be tricky without knowing the cell number. You could try to measure the size of your retinal explants and correlate the size to the efficiency. For organoids and neurosphyeres it is done in this approach.
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