calculation of Alkaloid Content
Where
Concentrations/ Absorbance of Atropine
5 µl = 0.0236
10 µl = 0.0345
20 µl = 0.0481
40 µl =0.0782
80 µl = 0.1345
160 µl = 03009
Where
Concentrations/ Absorbance of Sample
500 µl = 0.0489
Chukwuebuka Egbuna
Dear Mudasir Ahmad ,
I will suggest you prepare a standard curve of atropine from which you can determine the atropine equivalent alkaloid content of your sample. You may wish to have a look at the preliminary guide.
Chapter Phytochemical Test Methods: Qualitative, Quantitative and Pr...
Regards,
Egbuna
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