Hello friends, I am working on synthesis of sugar based silver nanoparticles. I confirmed its presence by the yellow colour formation. It was formed by mixing of 10 mL of 1 mM silver nitrate with 10 mL of 1 mM tri-sodium citrate solution containing 3 mM sugar. I want to calculate the final concentration of silver nanoparticles formed in the solution.
Hi, you can use UV-vis spectroscopy. use commercial available concentration and size of Ag known nano and then dilute to different concentrations and plot calibration curve . then run your unknown one and determine your concentation ; Also, you can use Atomic Absorption Spectrometer - Inductively coupled plasma mass spectrometry ( AAS and ICP-MS) instruments can be used to calculate the exact concentration of silver nanoparticals
read this it may help you
Hi, you can use UV-vis spectroscopy. use commercial available concentration and size of Ag known nano and then dilute to different concentrations and plot calibration curve . then run your unknown one and determine your concentation ; Also, you can use Atomic Absorption Spectrometer - Inductively coupled plasma mass spectrometry ( AAS and ICP-MS) instruments can be used to calculate the exact concentration of silver nanoparticals
read this it may help you
I concur. The exact concentration can be obtained either through atomic adsorption experiments or through ICP-MS techniques, depending on the concentration.
There is a discussion about this topic.
It may help.
https://www.researchgate.net/post/Can_we_calculate_the_concentration_of_Ag_NPs_silver_nanoparticles_using_the_absorbance_of_UV-Vis_spectrum_of_the_Ag_NPs_colloidal_solution
I recently measured the Ag concentration using ICP-AES after synthesis of AgNP using microalgae. I used NaCl solution to precipitate unreacted Ag+ as AgCl and then measured the stable AgNP in the supernatant. Here is the article (it has detailed method). ICP-MS would give you more accurate results though.
https://www.mdpi.com/1420-3049/24/1/98
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