I have determined part of (A) of a two part question where (A): the amount of cholesterol in your sample by reference to the standard curve, which is given to us. I am now unable to determine part (B) of said question which states I should:
Convert that (A) into the total cholesterol content of your 1g sample. CAREFUL you will need to take into account the fraction of the whole sample that you have loaded onto the HPLC.
Hi, Lisa,
I think the problem is solved this way:
From the peak area curve, also called calibration curve, you can determine the concentration of your analyte cholesterol. I think you have already done this. So you have the value mg/kg or any other concentration. But let us stay with mg/kg.
From this concentration you can then simply determine the amount of substance in 1g or ml
Example: Concetration 10 mg/l = 10mg /1000ml = 0.010 mg/ml
At least I understand the question to mean that you should specify the absolute amount of cholesterol in your sample
I hope this has helped you.
With kind regards
Joachim
Since you have a calibration plot, I do not think the calculation is hard. Using the peak area obtain the corresponding concentration followed by calculation of molality or molarity
Simple Chemistry is behind there
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