Would you be interested in contributing a chapter on Homemade biopesticides?

Dear Respected Researchers, Chapters are solicited on all areas of plant disease diagnosis; homemade biopesticides; indigenous practices in pest controls, scientific research etc. The book is...

07 August 2018 8,517 0 View

Anyone with an Optical Character Recognition software?

Dear Respected Researchers, Please anyone with useful link to a free software for converting scanned file in pdf to text files should send to me. I lost a very important file but have a hardcopy...

07 August 2018 9,576 5 View

Want to be part of the ongoing book project on phytochemistry?

We are coming towards the conclusion of this project on phytochemistry and you may wish to be part of it. An opportunity awaits you with very few topics desired to be included in the over 50+...

11 December 2017 6,723 3 View

Would you want to co-author a chapter in phytochemistry?

Apparently getting closer to the submission deadline on our ongoing collaborative book project in phytochemistry. I wish to invite you to to be part of it. If you are interested, please send me a...

10 November 2017 1,978 33 View

Want to contribute a book chapter on phytochemistry?

Dear Researchers, There is an ongoing project on phytochemistry with many collaborators now. If you are interested to join the team, please send me a mail at [email protected] for a list of...

08 September 2017 2,673 6 View

A textbook of phytochemistry: Structured into 5 sections. You want to be an author of the best phytochemistry textbook?

08 September 2017 9,747 37 View

No title

05 June 2017 1,771 10 View

How do someone cite some lines of statement that maybe you couldn't find the real contents?

If you find a recent publication (say work of 2015) that has some interesting sections credited to an author (original owner, say work of 1981) and you tried to access the original work/contents...

08 September 2016 9,781 7 View

What is the practical importance of Open Researcher and Contributor ID (ORCID)?

06 July 2016 9,125 2 View

How can I calibrate a UV-Vis spectrophotometer?

I came to understand that I am only doing a check of absorbance instead of internal calibration. Anyone with useful information should please help.

06 July 2016 7,492 11 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

16 February 2021 5,178 1 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

Why samples do not run in resolving gel?

Hello everybody, I have a problem with SDS-PAGE and I do not know why my samples stop at the top of the resolving gel and they do not run through it. The percentage of gel is 15% and the size of...

14 February 2021 9,116 6 View