How can I best recover viable cells from a brain tumor specimen that was frozen at the time of surgery?

25 April 2014 1 7K Report

We wish to recover cells from a brain tumor specimen that were frozen at the time of surgery and wonder if someone has a suggestion on a protocol.

Antoni Xavier Torres-Collado

I am sorry, but there are no viable cells on the frozen tissue. Next time you should get the cells before. You should follow a cell disgregation protocol (mechanic, trypsin, DNAse, etc) for brain.

Good luck next time

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