How to find out or construct(?) the right priors from data of previous experiment to use in a bayesian glm?

I have the following equation, Eating choice (Yes/No)~ Box type (A/B/C). So the question is whether a certain box type affects eating choice in groups of dogs. The eating choice of each member in...

10 February 2021 6,024 9 View

What are the ways to test the knowledge level of agricultural labourer about their day to day work?

what kind of test can I do for testing the level of knowledge about their daily operation?

03 February 2021 7,583 10 View

Why the bandgap of CH3NH3PbCl3 films are different for spin-coating method and electrospinning method?

The observed bandgaps are 3.8 eV and 3.4 eV for spin-coated and electrospun films, respectively. What are the possible reasons?

08 January 2021 5,646 6 View

Why monoethanol amine is used as flocculant?

I want to know the reaction mechanism of monoethanol amine used as flocculating agent for rerefining used lubricating oil. Here I have attached one research paper where MEA (monoethanolamine) is...

01 January 2021 5,681 2 View

Problem with GST-fusion protein elution- Can anyone help?

18 December 2020 4,559 6 View

How can I determine metal concentration of Used lubricating oil by AAS?

I am a junior research fellow in the department of Chemical Engineering, Nit Durgapur. I want to know that how can I determine various metal such as Fe, Cr, Mg, Mn, Pb concentration present in...

15 December 2020 9,887 3 View

Regarding the O 1s XPS pf phyllosilciates, the peak near 531 belongs to Oxygen vacancy sites or O bonded to the Si from the silicate tetrahedra?

In O 1s spectra, usually the peak near 531 eV belongs to oxygen vacancy sites in oxides, however, in silicates, these peaks are often reported to originate from O-Si. I am confused if the intense...

10 December 2020 9,496 2 View

What is the mol. weight of GFP-11 tag?

Hi, I am trying to find out the mol. weight of GFP 11 because it is tagged to my protein of interest which is 17kDa. GFP 11 has been tagged with my protein of interest while GFP 1-10 is tagged...

09 December 2020 1,863 1 View

How to PEGylate Fluorescntl Polystyrene Particle?

I have Fluorescent Polystyrene beads. I need to graft PEG on the surface of it. I am looking for a protocol to do that. Also need to know which experiment to perform to be sure about the grating.

01 December 2020 7,860 1 View

How to control for categorical independent variables (e.g., education) when doing regression discontinuity using STATA?

I am conducting a regression discontinuity design where the dependent variable is continuous while control variables are categorical (except the treatment variable). In the usual regression...

01 December 2020 583 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View