Hello everyone,
I have this idea but not so sure about its technical procedure, so I am grateful if you could give me some advice/guidance.
My goal is to make microhydrogel (or other biocompatible&bioorthogonal material) in simple 3D structure (sphere, cube, etc) that is about 5um in diameter and then attach e.coli on the surface of that microhydrogels. Those e.coli are biosensor I made with DNA circuits and it's important that they stay on the surface of microhydrogel.
As you could tell by now, I am inexperienced in synthesizing microhydrogels and also attaching e.coli. Ideas I got from some readings are: Synthesizing microhydrogel with microfluidics, Cover the microhydrogel with some carbohydrate-binding proteins so polysaccharides on e.coli can be linked and attached, and/or Synthesize e.coli somehow to make it very sticky.
I would really appreciate if you could comment or give reference on using microfluidics to synthesize those microhydrogel (or other better option) and what would be most probable candidates of proteins to cover the surface of microhydrogel so somehow e.coli naturally attach to the surface.
Thank you very much for your time!
Hi Mr. Seung Lee,
Micro-hydrogels can be prepared from a variety of biopolymers such as chitin, chitosan, alginate etc, by using different chemistry. The below work might be useful for you and its a very simple process. And since these are mostly polysaccharides, it might be easy for adhering the bacteria.
http://www.ncbi.nlm.nih.gov/pubmed/25893690
Thank you,
Arun
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