Which is the appropriate statistic test to examine the relation between students' affective state and academic performance?

Which test should I use to compare students' mood effects on academic performance? I want to compare mood measured on a likert scale (from point 1= very sad to point= 5 very happy) to three...

04 February 2021 1,907 5 View

How can you derive a and n parameters of the Van Genuchten equation for horticultural mediums?

Dear all, I am trying to figure out how to derive and n parameters of the Van Genuchten equation for compost samples taken from an urban farm. RETC has predefined values for these parameters for...

26 January 2020 7,843 3 View

I am trying to form microspheres with PCL and PVA using a mechanical stirrer but the polymers keeps forming clumps. What do i do to disperse them?

I prepared a 1% PVA, and dissolved 500 mg of PCL in 5 ml of DCM. Then I introduced the PCL solution into PVA while stirring at 2000 rpm for 2 hours, after which i place it on magnetic stirrer to...

15 April 2019 7,177 4 View

Can normal colon cell lines form spheroids?

I am growing spheroids from HT29 colon cancer cells, and I need some sort of control cells to be compared with the HT29. Now, I should grow the control cell into spheroids and then use these new...

30 October 2018 3,719 12 View

Please, can I get a research paper or an article on lipid extraction from ogbono and egusi using chloroform-methanol method ?

I am working on a project that involves lipid extraction using chloroform-methanol as solvents. I washed the product with water-methanol and about to use a rotary evaporator for evaporation. But...

10 May 2018 4,089 3 View

What is the effect of differentating 3T3-L1 preadipocytes in differentiation cocktail for 3 versus 7 days?

I noticed that some protocols use differentiation media for 2-3 days while others go for 5-7 days before changing to maintenance media. Why is that? Is an adipocyte grown in DM 3 days + MM 4 days...

08 December 2017 1,792 1 View

How to calculate sample size for a moderation study?

Hi, I am doing a study design and planning to do a moderation study. This is the simplest model. However, I am struggling with the calculation of sample size. How do I calculate the sample size...

23 April 2017 5,449 8 View

I want to take for the first time CHO cells in culture. How can I get 100% sterile glass Erlenmeyer flasks to use them again in the cell culture?

29 November 2015 9,661 3 View

How do I design primers that will distinguish between highly homologous transcripts?

10 August 2014 4,948 4 View

How can I get my ligation to work?

I've been attempting to subclone an insert from one vector to another by PCR amplification with primers that incorporate XhoI/EcoRI sites at the ends of the insert and then digesting the insert...

30 July 2014 4,230 6 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View