Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

What are the best references for an approach to teaching Differential Equations?

Differential equations are known to be potential tools for modeling and predicting recurrent phenomena in time and space, whether simple or more elaborate models. In this sense, how to approach...

18 December 2020 1,750 3 View

CIF file atoms without bonds in quantum espresso GUI?

Dear, I'm trying to use Burai 1.3 and quantum Vitas to create Quantum Espresso input from a crystal CIF file. However, when I opened the file (attached), I couldn't recognize the molecules, only...

27 November 2020 2,194 3 View

Are you a researcher or practicioner in the climate change adaptation field? Do you have knowledge or experience with Monitoring and Evaluation?

Dear Colleagues, My name is Sylvia Lima and I am conducting a survey as part of my Ph.D. research in evaluation of climate change adaptation success in coastal areas of developing countries with...

09 November 2020 6,434 3 View

What would be the best marine bioindicator to perform micronucleus counting?

I intend to evaluate mutagenesis and genotoxicity of marine pollutants and I think that freshwater plants and animals may not be the best bioindicators.

27 July 2020 2,818 1 View

Do you use any software to teach Engineering Economics (EE)?

I recommend the web application $AVEPI (System for Analysis of Economic Viability of Investment Projects: http://pb.utfpr.edu.br/savepi/index.php)

24 May 2020 1,120 3 View

Best strategy to transfect large genes in mammalian cells?

I have an insert ( ORF 6,9kb long) that I want to transfect in a human colon cancer cell line. I initially wanted to do with lentivirus, since I could have a stable expression of my gene and...

22 April 2020 3,277 3 View

How to insert Cre into an outbred strain?

I would like to transfer the Dat-Cre transgene (B6 background) into a outbred strain (SW) of mice. How can this be done? Do I start with a single breeding pair (Dat-Cre B6 x SW) and then for every...

02 February 2020 4,847 4 View

Which temperature should I use in Molecular dynamics (GROMACS) for a protein found in endoplasmatic reticulum?

Hi everyone, I'm trying to perform a MD simulation for a protein found in ER. In mdp file should I set: ref-temp= 300 K or 310K? gen-temp should be the same than ref-temp or 300...

22 June 2019 2,083 6 View

How to compare two RMSD plot (frame vs frame) from gromacs?

I have two proteins. One is wild-type and the other is a mutant. The RMSD plot (nm vs time) showed me mutant is far more unstable. But, when a plot the RMSD frame vs frame graphic it seems mutant...

28 May 2019 7,260 7 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View