Marian Freisleben

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Questions related from Marian Freisleben

DNA Gelelectrophoresis with agarose gel after ARDRA - artefacts?

For carrying out an Amplified Ribosomal DNA Restriction Anaysis (ARDRA) I had to separate the products of restriction-enzyme-digested cDNA in an agarose gel. The agarose concentration was 1% (w/v)...

20 November 2020 5,163 2 View

How can I apply degenerate primer in SerialCloner (2.6.1) to run a PCR?

I have to carry out an in silico PCR on the 16S-rRNA gene of 5 random bacteria strains. I would like to evaluate the resulting amplicon of different primer pairs. Because I can only use free...

15 November 2020 2,538 2 View